ABSTRACT
OBJECTIVE@#To investigate the protective role of Cardiospermum halicacabum (C. halicacabum) leaf extract on glycoprotein metabolism in streptozotocin (STZ)-induced diabetic rats.@*METHODS@#Diabetes was induced in male albino Wistar rats by intraperitonial administration of STZ. The C. halicacabum leaf extract (CHE) was administered orally to normal and STZ-diabetic rats for 45 days. The effects of C. halicacabum leaf extract (CHE) on plasma and tissue glycoproteins (hexose, hexosamine, fucose and sialic acid) were determined.@*RESULTS@#The levels of plasma and tissues glycoproteins containing hexose, hexosamine and fucose were significantly increased in STZ-induced diabetic rats. In addition, the level of sialic acid significantly increased in plasma and liver while decreased in kidney of STZ-induced diabetic rats. After administration of CHE to diabetic rats, the metabolic alteration of glycoprotein reverted towards normal levels.@*CONCLUSIONS@#The present study indicates that the CHE possesses a protective effect on abnormal glycoprotein metabolism in addition to its antihyperglycemic activity.
Subject(s)
Animals , Male , Rats , Analysis of Variance , Blood Glucose , Carbohydrate Metabolism , Diabetes Mellitus, Experimental , Blood , Drug Therapy , Metabolism , Fucose , Blood , Metabolism , Glycoproteins , Metabolism , Hexosamines , Blood , Metabolism , Hexoses , Blood , Metabolism , Hyperglycemia , Blood , Drug Therapy , Metabolism , Kidney , Chemistry , Metabolism , Liver , Chemistry , Metabolism , N-Acetylneuraminic Acid , Blood , Metabolism , Plant Extracts , Pharmacology , Plant Leaves , Chemistry , Protective Agents , Pharmacology , Rats, Wistar , Sapindaceae , ChemistryABSTRACT
OBJECTIVE@#To investigate the antihyperlipidemic effect of crude ethanolic extract of Melothria maderaspatana (M. maderaspatana) leaf (CEEM) on deoxycorticosterone acetate (DOCA)-salt hypertensive rats.@*METHODS@#A midscapular incision was made on each rat and the left kidney was excised after ligation of the renal artery. The surgical wound was closed using an absorbable suture. After one week recovery period, hypertension was induced by subcutaneous injection of DOCA-salt solution, twice a week, and the rats received a 1% sodium chloride solution as drinking water throughout the experimental period. CEEM or nifedipine was administered orally once a day for 6 weeks.@*RESULTS@#In DOCA-salt hypertensive rats, the level of plasma and tissues of total cholesterol (TC), triglycerides (TG), free fatty acids (FFA) and phospholipids (PL) significantly increased and administration of CEEM significantly reduced these parameters towards normality. Further, the levels of low density lipoprotein-cholesterol (LDL-C) and very low density lipoprotein-cholesterol (VLDL-C) significantly increased while high density lipoprotein-cholesterol (HDL-C) decreased in hypertensive rats and administration of CEEM brought these parameters to normality which proved their antihyperlipidemic action. Histopathology of liver, kidney and heart on DOCA-salt induced rats treated with CEEM showed reduced the damages towards normal histology.@*CONCLUSIONS@#These findings provided evidence that CEEM was found to be protecting the liver, kidney and heart against DOCA-salt administration and the protective effect could attribute to its antihyperlipidemic activities.
Subject(s)
Animals , Male , Rats , Cholesterol , Metabolism , Cucurbitaceae , Desoxycorticosterone , Toxicity , Ethanol , Pharmacology , Fatty Acids, Nonesterified , Metabolism , Hyperlipidemias , Blood , Drug Therapy , Hypertension , Blood , Hypolipidemic Agents , Pharmacology , Mineralocorticoids , Toxicity , Phospholipids , Metabolism , Phytotherapy , Methods , Plant Extracts , Pharmacology , Plant Leaves , Random Allocation , Rats, Wistar , Sodium Chloride, Dietary , Triglycerides , MetabolismABSTRACT
To determine the effect of combination of edible oils on blood pressure, anthropometric parameters, lipid profile, lipid peroxidative markers, antioxidant status and electrolytes in drug [nifedipine] taking patients with hypertension. In this study, patients were separated into 4 groups. Normal [n=14], hypertensive patients [n=38], 38 patients under medication with nifedipine were divided into 2 groups nifedipine control [n=12] and nifedipine + oil combination [sesame + sunflower oil] groups [n=26]. Sesame and sunflower oil combination was supplied to patients and instructed to use it as the only oil source for 45 days. Blood pressure and anthropometric parameters were measured at baseline and after 45 days. Lipid peroxidative markers, enzymatic and non-enzymatic antioxidants, lipid profile and electrolytes in blood were also measured. The study took place at Rajah Muthiah Medical College and Hospital, Annamalai University, Annamalainagar, India between January 2005 and December 2008. Nifedipine and oil-mix consumed patients significantly decreased the blood pressure, lipid peroxidative markers, lipid profile excluding the high density lipoprotein cholesterol [HDL-C], sodium, chloride, and increased enzymatic, non-enzymatic antioxidants, HDL-C and potassium levels when compared to nifedipine alone treated hypertensive patients. Nifedipine and oil-mix provided good protection over blood pressure and lipid peroxidation, and brought enzymatic and non-enzymatic antioxidants, lipid profile, and electrolytes towards normalcy in hypertensive patients